基于icpms的多重生物测定:一个有效的新兴平台
电感耦合等离子体质谱(ICP-MS)是im体育APP定量最通用的工具之一, 考虑到仪器对痕量im体育APP的灵敏度和低检测限, 以及它的线性动态范围和多重分析的便利性.
虽然分析物的化学或结构形式不能由 ICP-MS alone, 当与分离技术(通常是色谱分离)结合使用时, 连字符ICP-MS是一种特殊的色谱检测器. 在连字符应用中使用ICP-MS作为检测器提供了许多好处, including elemental selectivity, the ability to generate isotopic information, 以及同时识别多个im体育APP的能力. im体育APP的总浓度和游离浓度都可以测量, 常见的应用包括药代动力学(PK)和药效学(PD)研究, trace elements mass balance studies, and metabolite profiling, 以及微量im体育APP分析和im体育APP限量测试. Typically, 血浆或血清样本用于药代动力学(PK), drug clearance, half-life, and the determination of bioavailability.
Coupling ICP-MS with Chromatography: Hyphenated ICP-MS
电感耦合等离子体质谱仪器可以与广泛的色谱系统耦合. 连字符ICP-MS应用程序通常用于为LC-MS/MS提供补充数据. Specifically, 在LC-MS/MS无法检测到高度丰富的低电离效率代谢物的情况下, hyphenated ICP-MS can be employed, as the hyphenated system can produce sufficient data. 连字符ICP-MS最常用的应用是:
- Liquid chromatography (LC);
- High-performance liquid chromatography (HPLC);
- Reversed phase chromatography (RPC);
- Reversed phase ion pair chromatography (IPC), and
- Size exclusion chromatography (SEC).
Additionally, ICP-MS can be paired with:
- Ion exchange chromatography (IEC), and
- Normal phase chromatography (NPC).
而上述色谱系统被认为是更传统的耦合电感耦合等离子体质谱, ICP-MS也可用作气相色谱(GC)分离的检测器。. 这种分子物种形成的应用可能特别有用, 因为用于GC的其他特定im体育APP检测器无法达到相同的灵敏度, specificity, or wide range of elemental analysis. When compared to LC, GC typically offers higher resolution, 在某些情况和/或应用中,可能有利于生产非挥发性化合物的挥发性衍生物,专门用于GC分析.
Figure 1. Generic hyphenated ICP-MS system
The Utility of Hyphenated ICP-MS in Bioanalysis
When ICP-MS with hyphenated techniques is employed, organic compounds, organometallic compounds, and complex biomolecules, including nucleic acids, metal-proteins, and phospholipids, can be detected by means of their elemental content. As a result, 近年来,异原子标记蛋白质组学的研究取得了新的进展, 使用ICP-MS进行连字符分离,可以形成与蛋白质天然相关的具有生物学意义的杂原子(金属和半金属).
分析金属蛋白(含有金属离子辅助因子的蛋白质)对于充分了解金属在生物过程中所起的作用至关重要. 了解金属在生物过程中的作用对整个药物开发生命周期至关重要, 并且可以为生物标志物的选择和定量提供信息, 生物标志物是一种强大的工具,可以在药物开发的各个阶段实施, 从发现到临床试验分层和治疗药物监测(TDM).
ICP-MS为生物分子分析提供了独特的优势, 在复杂的样品基质中,不需要特定的目标标准来获得生物分子的绝对数量, given the hard ionization source of the instrument. Furthermore, 而蛋白质的绝对测定长期以来一直是一个挑战, 当同位素稀释分析(IDA)与稳定同位素作为生物标记物的非放射性标签结合使用时, ICP-MS能准确地进行生物分子定量. Additionally, 该连字符应用可作为免疫测定的替代检测技术.
开发高灵敏度的分析方法来检测生物基质中的im体育APP, optimal conditions and criteria should be met for:
- Accuracy;
- Specificity;
- Robustness;
- Precision;
- Detection and quantitation limit;
- Linearity, and
- Range.
当这些分析方法进行验证时,应遵循美国食品和药物管理局(FDA)生物分析方法工业验证指南.
Partnering with Element for Hyphenated ICP-MS Analysis
连字符ICP-MS方法有助于更好地了解杂质和潜在代谢物, 除了提供药物本身的定量信息, thereby ensuring and improving product safety, efficacy, and quality. 我们在成功开发和验证复杂和具有挑战性的大分子和小分子样品的最佳方法方面有着良好的记录, 采用广泛的连字符ICPMS方法, coupled with individualized sample preparation methods, including microwave digestion. Our consultative, 知识渊博的团队可以为广泛的应用设计精确的多路量化, 包括高通量生物标志物筛选和定量. Connect with a scientist today 讨论连字符ICP-MS方法是否适合您的需求.
Find related Resources
Learn more
![Connect with Element Life Sciences.](/-/media/images/sectors/pharmaceuticals/life-sciences-event-640x480.jpg?h=205&w=278&la=en&hash=EB2C951AEC78881AA47DB3C04C080AB3)
化学,制造和控制(CMC)咨询服务
We provide consulting services for Chemistry, Manufacturing, 制药和生物技术im体育平台app下载的产品开发和控制(CMC).
![Pharmaceutical CMC Bottles](/-/media/images/sectors/pharmaceuticals/pharma-cmc-product-development-480x640.jpg?h=205&w=278&la=en&hash=77DE6F82E974E6FF70795E77AC5FA630)
CMC Product Development Services
我们的CMC产品开发服务包括配方开发, parenteral and topical product development, microbiology testing services, and consultancy.
![DMPK and ADME laboratory services](/-/media/images/sectors/pharmaceuticals/av-dmpk-640x480.jpg?h=205&w=278&la=en&hash=9B7EAD76B668681E3A1E9DC5A867C30B)
DMPK & ADME Services
Element提供广泛的ADME和DMPK实验室服务, and we can accept both in vivo and in vitro samples.
![Subscriptions](/-/media/images/icons/subscriptions.png?h=205&w=278&la=en&hash=B7E77FC916DC0EBD64E1F7D84612213E)
Sign Up for Free Resources
访问Element的电子邮件订阅中心接收最新的im体育APP, technical whitepapers, case studies, webinars, and upcoming events.